The EZ Nucleosomal DNA Prep Kit is a streamlined procedure for the isolation of nucleosome-associated DNA. The kit includes reagents/procedures for the following: cell nuclei isolation, intact nuclei enzymatic digestion, and nucleosomal DNA purification. This kit includes two different enzymes for nucleosomal DNA preparation: Atlantis dsDNase and Micrococcal Nuclease. Enzymatic digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research€s proven spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes! For the isolation of nucleosome-associated DNA from mammalian and yeast cells. Ideal for use in nucleosome mapping studies.Storage Temperature: -20CStorage Conditions: FreezerApplicable For: All sensitive downstream applications such as qPCR and Next-Generation sequencing. Processing Volume: 106 cells Purity: Typical A260/A280 1.8 Sample Source: Nucleosome associated DNA isolation and purification from mammalian and yeast cells. Sample Storage: Eluted DNA should be stored at -20°C.Yield: Up to 25 μg total DNA can be eluted into 25 μl. For DNA 75 bp – 10 kb the recovery is 70-90%. For DNA 11 kb – 23 kb the recovery is 50-70%.Shipping Weight: 1.11 lbsShipping Dimensions: 7.00 x 5.00 x 4.50Research or further manufacturing use only, not for food or drug use.
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