5-hmC Glucosyltransferase, 200 Units, Zymo Research, Mfr#: ZE2027

The 5-hmC Glucosyltransferase is a highly active enzyme that specifically tags 5-hydroxymethylcytosine in DNA with a glucose moiety to yield glucosyl-5-hydroxymethylcytosine, which in turn can be used for sequence-specific, genome-wide, or global 5-hmC detection. Highly processive enzyme for specific modification of 5-hydroxymethylcytosine (5-hmC) with a glucose moiety. Ideal for locus specific and global quantification of hydroxymethylated DNA.Concentration: 2 U/μl Standard Reaction Time: 2 hours Storage: 5-hmC Glucosyltransferase for 12 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended with storage at -80°C.Unit Definition: One unit (U) is defined as the amount of enzyme needed to protect 1 μg of 5-hmC DNA Standard [D5405-3] from Csp6I restriction enzyme digestion via glucosylation in a reaction incubated at 30°C for 1 hour.Shipping Weight: 0.125 lbsShipping Dimensions: 5.30 x 3.30 x 0.50Research or further manufacturing use only, not for food or drug use.